Because of the ethical and societal considerations as well as the very high cost of animal testing, in vitro 3-D models that recapitulate normal or pathological human tissues are essential for understanding the intimate mechanisms of growth and for the development of new treatments. Spheroids or micro-tissues faithfully reproduce the organization of a tissue, recapitulating the cell-cell connections, cell-microenvironment and cellular heterogeneity found in the micro-regions of a tumor or organ. They are considered attractive models to assess the anti-proliferative activity of new drugs or genotoxic properties. The activity we offer is based on our strong expertise in the development of multicellular 3-D models from existing lines or from cells supplied by customers. We can support the assessment of a compound from 3-D cell culture, through the processing of 3-D models up to their three-dimensional exploration. We also propose culture in hypoxia and physoxia conditions of 3-D models grown either in suspension or in your scaffold.
How to explore and visualize the effects of new compounds? To fully exploit the capabilities of 3-D cell models, instead of an overall assessment, it is essential to preserve the 3-D regionalization level of analysis when investigating the effect of a drug. We provide a service to explore the dynamics of regionalization and the effects of new compounds in 3-D models. We will build for it on 2D and 3-D microscopy technologies like the most innovative light sheet microscopy, multi-photon (available on technological service platforms of ITAV). We also provide full imaging of your samples (up to 1 cm3): micro-tissues, organelles or fragments of bodies. These samples will undergo specific preparation according to the most appropriate imaging system.
3-D QUANTITATIVE ANALYSIS
How to characterize quantitatively the effects of new compounds? To get the most out of the experiments and make a decision with high confidence, we must be able to rely on numbers. We offer expert service in the characterization and quantification of all acquired data, including quantification of multiparametric images. We offer criteria for an objective assessment of the effects of molecules on multiple biological parameters of cells and tissues, such as the expression of target, viability, proliferation, cell cycle, 3-D organization, apoptosis, etc ...
Quantitative histological evaluation of the effects of a given compoun
Cell viability and proliferation assays
3D imaging and analysis of ultra-flat samples
3D characterization of preadipocytes
3D Quantification of multispheres growth in various scaffolds
3D Imaging of in vitro skin models
Evaluation of antiproliferative activity on 3D spheroid models
The leitmotif of the Imactiv-3-D company is to meet the challenges of evaluation of active molecules:
Imactiv-3D supports pharmas and biotech companies in their development strategy, study or research on innovative and reliable predictive models to help them in their decision making. Imactiv-3D relies for this on three complementary expertise: