On Tuesday, december 17, 2019, Aurélie Gomes successfully defended her thesis entitled "Evaluation of proliferation in tumor spheroids".
Her work, under a CIFRE agreement, is the result of 3 years of intense collaboration between the "Innovation for the study of 3D proliferation - IP3D" team at ITAV - Institut des Technologies Avancées en sciences du Vivant and IMACTIV-3D.
She was particularly interested in the evaluation of the proliferation in the 3D model of tumor spheroids and its regulation, in particular by oxygen. She has implemented experimental approaches to in toto antibody labelling, light sheet imaging and image analysis.
Her work has led to the development of methods and tools of interest in the study of cancer biology and response in anti-tumor pharmacology. Their application shows the importance of physioxia for the study of proliferation and the evaluation of drug response.
We are very happy that Aurélie Gomes continues the adventure with us. She will join IMACTIV-3D as a young doctor in January 2020 to share her expertise with our customers as R&D project manager in biology and imaging.
Start of APELYM 3D project
Apelym 3D is a new joint project between IMACTIV-3D and INSERM U1048 aiming to analyze the restoration of lymphatic function and adipose tissue by 3D imaging in context of lymphedema. Imactiv-3D will work to implement innovative tools for 3D imaging specific of the lymphatic network in adipose tissue and facilitate the characterization of the effectiveness of therapeutic targets in preclinical models of lymphedema. Alongside Imactiv-3D, two teams of INSERM U1048 are involved Florence Tatin (Team 13), coordinator of the project who works on the molecular regulation of lymphangiogenic factors and Philippe Valet (Team 3) who will bring its expertise on adipokines and adipocytes metabolism. While lymphatic capillaries have been well explored in several tissues, less is known about the deeper collecting lymphatic vessels.
Thanks to our complementary expertise, we will be able to explore this lymphatic network on adipose tissues to understand its involvement in lymphedema. Promising targets would be tested as potential actors of the lymphatic network regeneration. This collaborative project is supported by European Fund and Region Occitanie.
Imactiv-3D at Termis workshop on 3D Bioprinting for Cancer
The TERMIS-EU workshop is an event organized by INSERM Nantes and the University of Nantes on the 26th and 27th of August 2019 in Nantes (France).
The theme for the workshop is “3D bioprinting in cancer research”. This workshop is intended for medical students, doctors and researchers on oncology. Different themes will be addressed, including “3D bioprinting of tumor models, 3D models for cancer, 3D bioprinting and cancer microenvironment” and “3D bioprinting for tissue reconstruction”. Over the two days, practical workshops on 3D modeling and bioprinting are organized.
Imactiv-3D’s CEO, Jean-Michel Lagarde will make a pitch on Exploration and 3D quantification by light sheet imaging and image processing.
If you are interested in joining us for this workshop, you can register here: www.workshop-termis.eu
IMACTIV-3D at the AFSSI connexions in Lyon
Meet Imactiv-3D at the 7th edition annual meeting of preclinical research AFSSI Connexions on July 2-3, 2019 in Lyon (France).
Our CEO, Jean-Michel Lagarde, will be there to present Imactiv-3D’s solutions to support your preclinical research program with innovative imaging and image processing tools. We will be pleased to discuss about your projects and potential collaboration opportunities. Please feel free to contact us to schedule a meeting!
AFSSI Connexions will gather around 300 ecosystem stakeholders during two busy and productive days. Benefit from multiple opportunities to find the know-how, skills, and innovations that you need! Tuesday July 2: Thematic round table about: Business development strategies, Marketplace, International, Research Tax Credit, Growth and Competitiveness. Wednesday, July 3: Business convention day and business oriented presentations. Develop your business and relationships with your colleagues, centers of excellence, platforms and sponsors.
AIS Tours : 7th Antibody Industrial Symposium
Many thanks to all who were able to share with us during this event
Imactiv-3D at Meet2Win Oncology Partnering Convention
Meet Imactiv-3D at the Oncology Partnering Convention Meet2Win organized on May 21 and 22, 2019 in Bordeaux (France).
MATWIN organizes the 5th edition of this European Partnering Convention dedicated to open innovation, collaborative research and technology transfer.
MEET2WIN is a business convention combining flash project presentations, conferences, one-to-one business meetings as well as exhibition area.
Jean-Michel Lagarde, our CEO, will be there to discuss about the solutions that Imactiv-3D can offer to oncology research stakeholders.
IMALC Project Results
The IMALC project comes to an end! This research project was aiming to treat follicular lymphomas with anti-CD20 antibodies, and to develop quantitative imaging tools to evaluate their efficacy by light sheet microscopy.
To conclude, this transversal and interdisciplinary research project made it possible to:
- Characterize biologically relevant study models
- Model lightsheet imaging clinical observations, and maybe in the longer run, to explain why monoclonal antibodies treatments are less effective in patients with high-tumor burden follicular lymphomas or with an increased immune checkpoint expression.
This project is therefore part of a global research effort to better understand lymphomatous pathology and the mechanisms involved in therapeutic treatments in order to identify targets and anticipate resistance mechanisms. This represents a major challenge for a pathology that is currently considered incurable.
Imactiv-3D team has developed a quantitative analysis of the distribution and diffusion of MAb (Monoclonal antibody) on these 3D models of follicular lymphomas (MALC) based on 1) light sheet microscopy imaging of MALC after treatment with therapeutic ACMs and 2) the development of a custom methodology for efficient and automated image processing and analysis to quantitatively characterize the spatiotemporal biodistribution of MAbs within MALC. This project was based on the skills and expertise found in team N°9 of the CRCT (Toulouse Cancer Research Centre), on the imaging platform of the ITAV (Institut des Technologies Avancées du Vivant) and in the company IMACTIV3D.
Joint publication with INSERM in OncoImmunology
Imactiv-3D is very pleased to announce the publication of a new scientific article in the OncoImmunology Journal on follicular lymphoma, resulting from a collaboration with the Unit UMR1037 of the INSERM in Toulouse (France).
The article, entitled “Boosting γδ T cell-mediated antibody-dependent cellular cytotoxicity by PD-1 blockade in follicular lymphoma” is already available (DOI: 10.1080/2162402X.2018.1554175). This article presents data providing a rational treatment for follicular lymphoma by PD-1 blockade aimed at boosting γδ T cell anti-tumor functions in this pathology.
Imactiv-3D took charge of the image processing of 3D co-cultured MALC for characterization and quantification of the penetration of γδ T cells and monoclonal antibody (GA101).
New scientific publication in virology
Imactiv-3D is pleased to announce the publication of a new scientific article entitled “In vivo labelling of adenovirus DNA identifies chromatin anchoring and biphasic genome replication” in the Journal of Virology (DOI: 10.1128/JVI.00795-18) available here.
This study has been conducted in collaboration with the CNRS UMR5234 lab in Bordeaux (France) and with NeoVirTech. It shows that the real-time genome imaging system developed revealed hitherto uncharacterized features of adenoviral genomes in vivo. This system is capable of identifying new spatiotemporal aspects of the adenovirus life cycle and is potentially transferable to other viral systems with a double-stranded DNA phase.
Imactiv-3D team was involved in the characterization of viral replication centers in human cells and the image processing (denoising and deconvolution).
AFSSI : Les rencontres annuelles de la recherche préclinique
Thank you for the many contacts and discussions during this networking in Marseille
Meet Imactiv-3D at the 4th edition of the Oncology Business Convention Meet2Win organized by MATWIN in Bordeaux (France) on May 17th and 18th 2018.
MATWIN is a French platform supporting innovation in Oncology, subsidiary of Unicancer group (gathering the French Comprehensive Cancer Centers). Meet2Win is a partnering convention fully dedicated to open innovation in Oncology.
This event reunites more than 300 european representatives involved in oncology innovation (pharma, biotechs, startups, investors, researchers, cliniciants, TTOs etc). Jean-Michel Lagarde, our CEO, will be there to talk about our offer for oncology innovation.
IMLINFO (EFA281/16): Start of the European project
IMLINFO is a cross-border network of personalized immunotherapies against non-Hodgkin's lymphoma (NHL) involving 8 organizations located in Spain and France. Its main objective is to determine the efficacy of new immunotherapy treatments by creating a repository of non-Hodgkin's lymphoma (NHL) tumours associated with a three-dimensional (3D) culture platform.
SFIIC : Annual Seminar in Marrakech
During this seminar, Imactiv-3D presented 3D illustrations of skin structures ranging from adipocytes to reconstructed skin or bioimpression models.
Inexo : Symposium 2017 - Modèles Alternatifs
Imactiv-3D presented a complete 3D cell culture, imaging and quantification process for the evaluation of the cytotoxicity of compounds.in Montpellier
Start of IMALC Project on Follicular Lymphomas
The project aims to develop quantitative imaging tools for the evaluation of treatment efficacy of follicular lymphomas using light sheet microscopy. To identify a treatment and evaluate it as objectively as possible requires methods capable of extracting, quantifying and processing information relating to numerous markers. Coupling light sheet microscopy with quantitative image analysis methods directly addresses this need.
This project is co-financed by the ERDF and the Occitanie Region. Imactiv-3D works with two labs: ITAV (CNRS USR3505) and CRCT (INSERM U1037).
The IMALC project aims to carry out an analysis of quantitative distribution and diffusion of monoclonal antibodies (mAbs) on 3D models of lymphomas, based on light sheet microscopy imaging of MALC after treatment with therapeutic monoclonal antibodies and the development of a custom high-performance methodology, automated image processing and analysis to characterize quantitatively the spatiotemporal biodistribution of mAbs within MALCs. Image: Visualization of a MALC using light sheet microscopy 24h-post treatment with GA101. The color gradient (from blue to white) shows the localization of the GA101 intensity.
Imactiv-3D first scientific publication
Imactiv-3D is very proud to announce its first scientific publication in the Oncology Letters!
This scientific article, entitled "Evaluation by quantitative image analysis of anticancer drug activity on multicellular spheroids grown in 3D matrix" is available here (DOI: 10.3892/ol.2016.5221).
Our team developed a new procedure to visualize, analyze and quantify the pharmacological activity of drugs on 3D multicellular spheres grown in a physiological matrix.
The aim of this study was to propose a robust and efficient imaging and analysis pipeline to assess with quantitative parameters the efficacy of a particular cytotoxic drug. HCT116 colorectal adenocarcinoma tumor cell multispheres were observed with structured illumination 3D microscopy and the images obtained were processed with custom analysis tools.
We were thus capable of automatically detecting spheres in a large volume of matrix in 96-well plates. This procedure was used to evaluate drug efficacy in HCT116 spheroids treated with different concentrations of topotecan, a DNA topoisomerase inhibitor. This work demonstrates that quantitative image analysis is an effective mean to evaluate and quantify the cytostatic and cytotoxic activities of anticancer drugs on 3D multicellular models grown in a physiological matrix.
Establishment of the company
Hosting of the company within the CNRS in Toulouse (National Centre for Scientific Research).