Categories:
Keywords:
Pharmacological evaluation of anticancer drugs using 3D pre-clinical in vitro model provides invaluable information for the prediction of in vivo activity. The work reported here presents an efficient and robust imaging and analysis process to assess with quantitative parameters the efficacy of a cytotoxic drug. To illustrate our service offer, we present the quantitative evaluation of dose-dependent Topotecan cytotoxicity on HCT116 colon adenocarcinoma tumor cells multispheres grown in 3D.
Our workflow involves 3D microscopy with structured illumination, image processing and feature extraction performed with custom analysis tools. Our procedure allows automatic detection of spheres in a large volume of matrix in 96-wells plate. The resulting quantification of morphometric parameters such as cluster’s size distribution shows variation correlated with Topotecan concentration.
Future developments aim to integrate more channels of fluorescence in order to study markers of proliferation or apoptosis. The image represents an example of a Hoechst
dye in blue for the recognition of cell clusters and an EDU dye in green for the proliferative aspect.
This procedure allows by using quantitative image analysis to evaluate and quantify the cytotoxic activity of anticancer drugs on 3D multicellular models, and can be applied to multi-parametric analysis to address customers’ specific needs.

CytotoxicityMulti-Spheroid

Pharmacological evaluation of anticancer drugs using 3D pre-clinical in vitro model provides invaluable information for the prediction of in vivo activity. The work reported here presents an efficient and robust imaging and analysis process to assess with quantitative parameters the efficacy of a cytotoxic drug. To illustrate our service offer, we present the quantitative evaluation of dose-dependent Topotecan cytotoxicity on HCT116 colon adenocarcinoma tumor cells multispheres grown in 3D. Our workflow involves 3D microscopy with structured illumination, image processing and feature extraction performed with custom analysis tools. Our procedure allows automatic detection of spheres in a large volume of matrix in 96-wells plate. The resulting quantification of morphometric parameters such as cluster’s size distribution shows variation correlated with Topotecan concentration. Future developments aim to integrate more channels of fluorescence in order to study markers of proliferation or apoptosis. The image represents an example of a Hoechst dye in blue for the recognition of cell clusters and an EDU dye in green for the proliferative aspect. This procedure allows by using quantitative image analysis to evaluate and quantify the cytotoxic activity of anticancer drugs on 3D multicellular models, and can be applied to multi-parametric analysis to address customers’ specific needs.

3D vizualisation of the rat liver vascular network

AnimalsRatLiverVascular Network

Images taken by wide-field light sheet microscopy, after sample transparisation, without any staining. Automatic vascular network recognition by image processing.

3D vizualisation of a mouse lung

AnimalsMouseLung

Images taken by wide-field light sheet microscopy, after sample transparisation, without any staining.

3D exploration and quantification for the study of heart disease

AnimalsMouseHeart

Images taken by wide-field light sheet microscopy, after sample transparisation, without any staining. Automatic ventricule recognition by image processing.

×
×